Genome Projects and Sequencing Technologies · Zoology

Illumina sequencing is based on the principle of:

  1. Dideoxy chain termination during synthesis
  2. Sequencing by synthesis
  3. Sequencing achieved by successive ligation
  4. Direct sequencing of native RNA molecules
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Correct answer: Sequencing by synthesis

Illumina technology uses sequencing by synthesis. Fluorescently labeled nucleotides are detected as they are incorporated.

Difficulty: Medium Question 9 of 20

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