DNA Fingerprinting and Molecular Markers · Zoology

Restriction Fragment Length Polymorphism (RFLP) analysis depends on:

  1. Polymerase chain reaction amplification of the region
  2. Restriction enzyme digestion
  3. In vitro transcription of RNA from the template
  4. Analysis of overall protein expression levels
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Correct answer: Restriction enzyme digestion

RFLP relies on restriction enzymes cutting DNA at specific sites. Variations in these sites produce fragments of different lengths.

Difficulty: Medium Question 11 of 20

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