Enzymes: Classification, Kinetics and Inhibition · Zoology

Non-competitive inhibition affects enzyme activity by:

  1. Blocking the active site directly
  2. Reducing the substrate binding rate
  3. Altering enzyme conformation
  4. Competing with the substrate
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Correct answer: Altering enzyme conformation

Non-competitive inhibitors bind to an allosteric site separate from the active site, inducing a conformational change that reduces catalytic efficiency. Because substrate binding is unaffected, Km remains unchanged while Vmax decreases.

Difficulty: Medium Question 12 of 20

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