Microbial diagnostics · USMLE Step 1

A patient with suspected cryptococcal meningitis undergoes lumbar puncture. Which diagnostic test is most sensitive?

  1. India ink stain of CSF
  2. Cryptococcal antigen assay
  3. CSF culture on Sabouraud agar
  4. Gram stain of CSF
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Correct answer: Cryptococcal antigen assay

Cryptococcal antigen testing is highly sensitive, even with low organism burden. India ink stain may miss cases with low fungal counts.

Difficulty: Medium Question 12 of 20

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