Urinalysis · Pathology

The 'Specific Gravity' measured by a dipstick is based on which principle?

  1. The pKa change of a polyelectrolyte with ionic concentration
  2. The bending of light by dissolved solutes (refractive index)
  3. The total mass of dissolved solids relative to pure water
  4. The frequency of transmitted light wavelength through the sample
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Correct answer: The pKa change of a polyelectrolyte with ionic concentration

Dipstick specific gravity measurements rely on the release of hydrogen ions from a polyelectrolyte in proportion to the number of cations in the urine. This change in pH is detected by a bromothymol blue indicator on the reagent pad.

Difficulty: Medium Question 14 of 20

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