Semen Analysis · Pathology

The 'Hypo-osmotic Swelling' (HOS) test is used to evaluate:

  1. Sperm concentration
  2. The integrity of the sperm plasma membrane
  3. Acrosome reaction capability
  4. The presence of fructose
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Correct answer: The integrity of the sperm plasma membrane

The HOS test identifies live sperm with functional membranes. When placed in a hypo-osmotic solution, live sperm with intact membranes will absorb water and their tails will swell/curl, whereas dead sperm will not.

Difficulty: Medium Question 19 of 20

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