Glycolysis: Pathway and Significance · NCERT Biology

Which enzyme catalyzes the phosphorylation of glucose to glucose-6-phosphate?

  1. Phosphofructokinase
  2. Pyruvate kinase
  3. Hexokinase
  4. Enolase
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Correct answer: Hexokinase

Hexokinase is the enzyme that initiates glycolysis by phosphorylating glucose at the expense of one ATP molecule. This step traps the glucose molecule inside the cell as a charged phosphate ester.

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