Dark Reactions: Calvin Cycle and C3 Pathway · NCERT Biology

In which part of the chloroplast do the 'Dark Reactions' or biosynthetic phase occur?

  1. Thylakoid lumen
  2. Grana
  3. Stroma
  4. Inner membrane space
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Correct answer: Stroma

The dark reactions involve enzymatic steps that do not directly require light but depend on the products of light reactions (ATP and NADPH). These enzymes are located in the fluid stroma of the chloroplast.

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