Periosteum · Histology

Which staining method is most commonly used to visualize the collagenous fibrous layer of the periosteum in histology?

  1. Hematoxylin and Eosin
  2. Silver impregnation stain
  3. Sudan Black B stain
  4. Periodic Acid-Schiff
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Correct answer: Hematoxylin and Eosin

H&E is the standard stain used to visualize bone tissue. The collagen in the fibrous periosteum stains pink (eosinophilic), while the nuclei of the fibroblasts and osteoprogenitor cells stain blue/purple (basophilic).

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