Plant Tissue Culture · Botany

What is the primary function of surface sterilization of an explant?

  1. To induce division in explant surface cells
  2. To remove pathogens embedded within plant tissues
  3. To eliminate microorganisms present on the surface
  4. To increase the plant's overall rate of photosynthesis
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Correct answer: To eliminate microorganisms present on the surface

Surface sterilization using agents like sodium hypochlorite or mercuric chloride is essential to remove bacteria and fungi from the exterior of the plant tissue. Failure to do so leads to microbial overgrowth that competes with the explant for nutrients.

Difficulty: Medium Question 3 of 20

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