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DNA Structure · Botany

Denaturation of DNA refers to:

  1. The enzymatic synthesis of new complementary DNA strands.
  2. The separation of the two strands due to the breaking of hydrogen bonds.
  3. The irreversible breaking of phosphodiester bonds in the sugar-phosphate backbone.
  4. The enzymatic removal of introns from the DNA sequence.
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Correct answer: The separation of the two strands due to the breaking of hydrogen bonds.

Denaturation (or melting) occurs when high heat or chemicals break the hydrogen bonds between bases, causing the double helix to separate into single strands. This process is reversible (renaturation).

Difficulty: Medium Question 19 of 20

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